Aziz Mahmoud Aziz Tumeh

Aziz Mahmoud Aziz Tumeh

PGSB Fellow
Arab American University-Palestine

Specialization: Structural Biochemistry and Biophysics

German supervisor: Prof. Jorg Labahn

Palestinian supervisor: Prof. Hilal Zaid

Project Title: Structural Characterization of Proapoptotic Par-4

Project Purpose: Par-4 is a 332 amino-acid proapoptotic protein with tumor suppressor activity. It is predominantly unstructured and contains two important domains. It is well known for its selective induction of apoptosis in cancer cells and is attributed to the N-terminal SAC domain. The structure of the Cterminal coiled coil (CC) domain, which is mainly involved in many of its interactions, has been determined. Par-4 is a nucleo-cytoplasmic shuttling protein containing a nuclear localizing sequence (NLS) in the SAC domain and a nuclear export sequence (NES) in the CC domain. 
The  current project is aimed at determining the structure of extended regions from CC-domain to include the SAC domain of Par-4 . This may require the expression of protein in insect cells for PTM, and include some of the interacting proteins like autophagy receptor p62/SQSTM1, nuclear export receptor CRM1, or GRP78 protein to induce structure and stability of Par-4 upon complex formation.
 This project has the possibilities to explore molecular cloning, microbiology (protein expression), electrophoretic and chromatographic techniques (protein purification), biophysical characterization (mass spectroscopy, circular dichroism spectroscopy, fluorescence spectroscopy, etc.) and 3D structure determination (X-ray crystallography).